Members

Morad ROUDBARAKI, PhD, HDR

morad.roudbaraki@univ-lille.fr

Phone: +33.320.33.64.21

Pascal MARIOT, PhD, HDR

pascal.mariot@univ-lille.fr

Phone: +33.320.33.71.93
 

Pascal Mariot obtained his PhD in Life Sciences (Neurosciences and Pharmacology) from the University of Bordeaux II, in September 1993. During his PhD, he focused his studies on the role of ion channels and proton transporters in the secretion of pituitary hormones. He then joined in September 1993 the Department of Physiology at University College London for 3 years (1993-1996) , where he studied GTP-dependent mast cell degranulation using membrane capacitance monitoring. From 1996 to 1997, he obtained a post-doctoral position at the University Catholique de Louvain (UCL, Belgium) in the department of endocrinology (Brussels, Hopital Saint Luc), and worked on the role of voltage-dependent Ca²⁺ channels and ATP-dependent K⁺ channels in insulin secretion. He is a permanent member of the Laboratory of Cell Physiology since 1997, where he works as an Associate Professor to investigate the mechanisms, and noticeably the role of Ca²⁺ in prostate cancer progression.

V'yacheslav LEHEN'KYI, PhD, HDR

vyacheslav.lehenkyi@univ-lille.fr

Phone: +33.320.33.70.78

Dr. V'yacheslav LEHEN'KYI is an alumni of the University of Taras Shevchenko, Biological faculty, Department of Biochemistry. In October 2001 he obtained his PhD in Pharmacology in the Laboratory of Experimental Therapy, at the Research Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine. His PhD work concerned the sensitivity of the vascular smooth muscle contractile machinery to the NO action. In 2002-2003 he did his first postdoc in the Laboratory of Molecular and Cell Cardiology headed by Dr. Rodolphe Fischmeister, at the University of Paris XI where he studied small GTPase Rac1 and CDC42 effects of cardiac hypertrophy. His second postdoc he continued in Kyiv where he studied the cGMP-independent effects of NO on the arterial smooth muscles. Dr. V'yacheslav LEHEN'KYI joined our Laboratory in 2004 as a postdoctoral researcher to study the mechanisms where such TRP calcium channels as TRPV6, TRPV2, TRPC1-6, in adition to the calcium channel Orai1 and ER calcium sensor STIM1, are involved in the prostate cancer. During 2004-2015 his second research project related to the role of the above channels in the skin homeostasis such as a balance of proliferation, differentiation, migration and senescence. Today, Dr. V'yacheslav LEHEN'KYI is responsible for the research Axis:  
EXTRAPEP (project funded by SATT du Nord at the level of 660000€ for 2 years)

• Role Of The Calcium Channel TRPV6 In The Development Of The Bone Metastasis In Prostate Cancer (project funded by “Ligue Contre Cancer” at the level of 40 000€ /year for 3 years)
• Role of the TRPV6 and KCa3.1 channels in pancreatic ductal carcinoma (PDAC) (projects funded by “Ligue Contre Cancer”, 20 000€ and our LAI).

Curriculum Vitae

Publications

https://pro.univ-lille.fr/vyacheslav-lehenkyi

Christophe TASTET, PhD

christophe.tastet@univ-lille.fr

Phone: +33.320.33.63.45

Christophe Tastet obtained his PhD in Proteomics from the Ecole Normale Supérieure Agronomique de Rennes in May 2000. During his PhD (INRA), he focused on the application of a putative fatty-acid binding protein to discriminate serologically two European quarantine phytoparasites from other species by means of two-dimensional electrophroregram (2D IEF/SDS-PAGE) computed analysis. In 2001, he was a postdoctoral fellow in the laboratory of Dr Thierry Rabilloud at the Commissariat à l'Energie Atomique (CEA, Grenoble) where he has specialized in protein solubilisation and extraction, with a focus on membrane proteins. Then, he completed a second postdoctoral fellowship in proteomics applied to Parasitolgy (Malaria) at the CNRS Montpellier under the supervision of Drs Henri Vial and Jean-françois Dubremetz. Recruited in 2005 as Assistant professor (Maître de Conférences) in the laboratory INSERM u908 "Signalisation des facteurs de croissance dans le cancer du sein - Protéomique fonctionnelle" managed by Pr Hubert Hondermarck; he joined our laboratory (INSERM u1003) in 2014 to study the protein interactions between the plasma membrane and the ER (or mitochondrial) by means of cationic colloidal silica cell surface proteome analysis (prostate cancer cells).

George SHAPOVALOV, PhD, MCF

george.shapovalov@inserm.fr

Phone: +33.320.33.64.21

George Shapovalov has done his PhD at California Institute of Technology, applying biophysical methods to study mechanosensitive channels at single- channel methods, working on isolated as well as synthesyzed proteins reconstituted in artificial liposomes. He continued his activities as a postdoc in University of Geneva, studying role of various ion channels in Duchene Muscular Dystrophy. He entered our laboratory as a postdoc in July of 2010 and became as Associate Professor in Sept 2014. His research covered topics ranging from studying role of TRP channels in prostate cancer progression to development of approaches to study intracellular ion channels and their regulation by endogenous protein partners.

Charlotte DUBOIS, PhD

charlotte.dubois@inserm.fr

+33.320.33.70.78

Charlotte DUBOIS obtained her Ph.D. in Biological Sciences at the University of Lille. During her PhD (sept 2011-dec 2014), she conducted 2 projects: (1) the role of ORAI1/3 (ARC) channels during prostate cancer progression and (2) the characterization of Thapsigargin analogues (i.e. ASP-8ADT also known as G-202(R) (Mipsagargin). Then she moved to the University of Leuven (Belgium) to join the laboratory of Pr. Peter CARMELIET (Laboratory of vascular biology CCB, VIB KULeuven) to study the metabolism of endothelial cells during physiological and pathological angiogenesis. From October 2015 to September 2018, she has depicted a new non-metabolic role of the glutamine synthetase (GLUL) during angiogenesis. She has joined back our lab in November 2018 to conduct a project founded by the Fondation de France and I-SITE Université de Lille. Using an oncogram strategy she aims to (1) predict efficiency of (combined) chemotherapies to offer a personalized medicine for each patient and (2) identify (calcium-dependent) molecular mechanism(s) involved in therapeutic escape of solid cancers (such as prostate). In November 2021 she has been recruited by INSERM as CRCN (full-time researcher). Her project will focus on the different roles of ORAI1/3 channels within the prostate tumor microenvironment, with a specific focus on the neuro-angiogenesis process.

Roman SKRYMA, PhD, HDR

roman.skryma@univ-lille.fr

+33.320.33.63.82

Alessandra FIORIO PLA, PhD

Full Professor in Physiology

Dept. Life Sciences and System Biology University of Torino, Italy

alessandra.fiorio[chez]unito[point].it

Phone: +33.320.33.68.38

Alessandra's research activity is focused on cell physiology with particular interest for Ca²⁺ channels characterization and their involvement in angiogenesis and tumor progression by combining calcium imaging and live cell imaging techniques with cell biology. She is mainly interest on the role of TRP channels in the different pathophysiological role in tumor vascularization and particularly in cell migration and tumor invasion.

Dr. Jean-Louis BONNAL, MD

Saint Philibert - Saint Vincent de Paul Hospitals

bonnal.jean-louis[chez]ghicl[point].net

Phone:  +33.320.22.50.76

Lilian MUTELICA, MD

Saint Philibert - Saint Vincent de Paul Hospitals

mutelica.lilian@ghicl.net

Phone: +33.320.22.50.76

Nicolas VAMOUR, MD

Saint Philibert - Saint Vincent de Paul Hospitals

vamour.nicolas@ghicl.net

Phone: +33.320.22.50.76

Tanguy RINGEVAL, MD

Saint Philibert - Saint Vincent de Paul Hospitals

ringeval.tanguy@ghicl.net

Phone: +33.320.22.50.76

Dheeraj KANNANCHERI PUTHOORU, PhD

dheeraj.kannancheri-puthooru@inserm.fr

Phone: +33.320.33.64.23

Dheeraj obtained a BS-MS dual degree with a major in Biology and a minor in Chemistry from the Indian Institute of Science Education and Research (IISER) Thiruvananthapuram, India. He completed his Ph.D. in our laboratory, where his thesis addressed the role of calcium channels in prostate cancer drug resistance. Actually, Dheeraj's work focuses on elucidating the unique drivers of CRPC-NE progression and the potential involvement of calcium channels. By integrating multidisciplinary approaches on in vitro and in vivo models, including primary cells and patient-derived xenografts, he aims to uncover the role of calcium channels in the mechanisms underlying neuroendocrine transdifferentiation, treatment resistance and tumor aggressiveness.

Maya YASSINE, PhD

maya.yassine@inserm.fr

Phone: +33.320.33.62.70

Maya had MS in Biological Science from the American University of Beirut, where she was selected by Erasmus Mundus International scholarship for excellence (2010-2014) tu pursue a PhD in cell and molecular biology in our lab. her PhD project involved the role of calcium-permeable channels in the regulation of autophagy in prostate cancer. After investing her studies as a biology professor in higher academic institutions, she joined back our lab in 2021 as a post-doc to investigate the role of calcium in the control of autophagy and cell death for future development of novel autophagy modulators targetinf ion channels for cancer treatment.

Aurélien HAUSTRATE, PhD

aurelien.haustrate@inserm.fr

Phone: +33.320.33.64.23

Clément CORDIER

clement.cordier@inserm.fr

Phone: +33.320.33.70.78

Clément has a background in Biology and Oncology (MSc in Health Biology). His research focuses on the role of the TRPV6 calcium channel in the development of bone metastases in castration-resistant prostate cancer (CRPC), with the aim to elucidate the molecular mechanisms involving TRPV6 and to develop monoclonal antibodies to inhibit metastatic progression. His recent work showed that a new therapeutic strategy can potentiate the effect of anti-TRPV6 antibodies, effectively preventing bone metastases and improving overall survival in preclinical models.

Anastasiia DANSHYNA

anastasiia.danshyna@univ-lille.fr

Phone: +33.320.33.62.70

Anastasiia is currently investigating the role of calcium ion channels involved in aggressive pancreatic and prostate cancer phenotypes, with a focus on tumor microenvironment, under the direction of Prof. Natalia Prevarskaya and Prof. Yaroslav Shuba, co-supervised by Dr. Valerio Farfariello.

Sofia TITAH

sofia.titah@inserm.fr

Sophia is currently doing her research on the calcium signature of the immunological synapse in the presence of the PD-1/PD-L1 axis in acute myeloid leukemia and the contribution of microfluidics, under the supervision of Dr. Loïc Lemonnier Dr. Yasmine Touil (CANTHER Unit, OncoLille).

Valerio FARFARIELLO, PhD

Research Engineer

valerio.farfariello@univ-lille.fr

Phone: +33.320.33.62.70

Valerio Farfariello obtained his PhD in Immunological Sciences from the Sapienza University, Rome (Italy) in March 2012. During his PhD, he studied the role of Ca²⁺-permeable channels (TRPV family) in the maturation of thymocytes. In this period, he also studied the role of TRPV channels in the proliferation and malignant phenotype of brain, bladder and prostate cancer cells. Valerio joined our laboratory in January 2014 as a postdoctoral fellow to study the role of Ca²⁺-permeable channels in senescence and senescence escape occurring during prostate carcinogenesis. He is also responsible of a project dedicated to the comprehension of the mechanisms of chemotherapy resistance caused by the senescent microenvironment with a focus to Ca²⁺ channels.

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